


Generally, I’m of the opinion that permanent manipulations to an image should be avoided. Here’s why… Why this may not be a great idea What you probably shouldn’t do, is use it in a figure for publication or on a poster. Hit OK, and you can now copy the image into a slideshow presentation or report. Run the command to be presented with the following (fairly self-explanatory) dialog: With a calibrated image, adding a scale bar couldn’t be simpler. NOTE: I’m not going to deal with calculating the calibration information here, but it will probably be the topic of another post. If you’re less outgoing and want to stick to micrometres, “um” or “micron” are acceptable and will automatically be converted into “μm”.

You can pick any Unit you want, for example the much under-used (and definitely not made up) micro-dolphin. If your image is uncalibrated, enter the calibration information here. You can also find the calibration info by opening up the Properties of the Image, where you’ll get a dialog not too dissimilar to this: If an image has calibration information (above, right), the calibrated dimensions of an image are displayed with the units (65.02μm) followed by the pixel dimensions (256×256). Example of an uncalibrated (left) and a calibrated (right) image in Fiji.
